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Sådan Undersøgelse Basement Membrane stivhed som en biofysisk Trigger i prostatakræft og andre aldersrelaterede patologier eller metaboliske sygdomme
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
DOI:

13:18 min

September 20, 2016

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Chapitres

  • 00:05Titre
  • 01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
  • 04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
  • 06:27Immunofluorescent Microscopy Analysis
  • 09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
  • 10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
  • 12:02Conclusion

Summary

Traduction automatique

Her forklarer vi en protokol til modellering af biofysiske mikromiljø hvor krydsbinding og øget stivhed af basalmembranen (BM) induceret af avancerede glykering slutprodukter (alder) har patologisk relevans.

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