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Hvordan studere basalmembran Stivhet som Biofysikalsk Trigger i prostatakreft og andre aldersrelaterte patologi eller metabolske sykdommer
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
DOI:

13:18 min

September 20, 2016

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Chapitres

  • 00:05Titre
  • 01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
  • 04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
  • 06:27Immunofluorescent Microscopy Analysis
  • 09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
  • 10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
  • 12:02Conclusion

Summary

Traduction automatique

Her forklarer vi en protokoll for modellering av biofysiske mikromiljøet der kryssbinding og økt stivhet i basalmembran (BM) indusert av avanserte glycation sluttprodukter (aldre) har patologisk betydning.

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