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Immunologie et infection
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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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Immunologie et infection
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Journal JoVE Immunologie et infection
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

DOI:
10.3791/54293-v

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08:21 min

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July 28, 2016

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, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Chapitres

  • 00:05Titre
  • 00:45Preparing Giant Unilamellar Vesicles (GUVs): Part 1
  • 03:25Preparing GUVs: Part 2, and Harvesting the In Vitro Translation Reaction
  • 05:47Harvesting the GUVs and Binding Assay
  • 06:43Results: HIV-1 Gag Binding to GUVs
  • 07:32Conclusion

Summary

Traduction automatique

Traduction automatique

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

Tags

Keywords: HIV-1 GagGiant Unilamellar Vesicle (GUV)Membrane BindingLipid CompositionVirostructural ProteinsElectroformationITO-coated SlidesLipid Film FormationChloroformTemperature Control

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