Journal
/
/
Bereiding van formaline gefixeerde paraffine-ingebed weefsel Cores voor zowel RNA en DNA-extractie
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
DOI:

08:30 min

August 21, 2016

, , , , , , , , , ,

Chapitres

  • 00:05Titre
  • 00:57Tissue Coring
  • 02:40Deparaffinization of the FFPE Tissue Cores
  • 03:45Homogenization of the Deparaffinized Cores
  • 05:07Digestion with Proteinase K and Nucleotide Separation
  • 06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
  • 07:50Conclusion

Summary

Traduction automatique

Deze gewijzigde extractie protocol verbetert RNA en DNA opbrengst van meer doelgerichte regio's van belang in histopathologische weefsel blokken.

Vidéos Connexes

Read Article