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Un método de aislamiento de células reconocidas a través del vidrio funcionalización de la superficie
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Chapitres
- 00:05Titre
- 01:03APTES and DSB Functionalization
- 03:58Streptavidin Functionalization
- 04:58Cell Capture and Release
- 07:36Results: Functionalized Cell Capture of MCF7GFP and RAW Macrophage Cells
- 09:18Conclusion
This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.
