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Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
DOI:

10:51 min

August 30, 2016

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Chapitres

  • 00:05Titre
  • 01:24Growth of Microengineered Intestinal Villi in the Gut-on-a-Chip Device
  • 03:45Formation of Microengineered Caco-2 Villi in the Gut-on-a-Chip
  • 05:55Application of Mechanical Deformations Mimicking Peristalsis-like Motions
  • 06:37Host-gut Microbiome Co-culture in a Gut-on-a-chip Microdevice
  • 09:07Results: Emulating the Human Intestinal Host-microbiome Ecosystem
  • 09:44Conclusion

Summary

Traduction automatique

We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

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