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민감한 면역를 사용하여 시토신의 수정 된 형태의 검출
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
DOI:

07:13 min

August 16, 2016

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Chapitres

  • 00:05Titre
  • 00:47Fixation and Permeabilization of Cryo- and Microtome Sections
  • 01:34Immunostaining for Oxi-5mC Derivatives
  • 05:16Results: Immunohistochemical Analysis of 5hmC and 5caC Distributions in the Adult Mouse Brain Tissues
  • 06:12Conclusion

Summary

Traduction automatique

Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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