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肉認証にペプチド応用のMRM質量分析法を用いて混合物中の種の決意と定量
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
DOI:

09:26 min

September 20, 2016

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Chapitres

  • 00:05Titre
  • 00:54Proteolysis and Analysis of Reference Myoglobins
  • 05:14Preparation and Analysis of Calibration Samples
  • 07:57Results: Example Outcomes for a Mixture of Horse in Beef
  • 08:33Conclusion

Summary

Traduction automatique

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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