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Ontwikkeling van een antigeen-gedreven Colitis Model naar Presentatie van antigenen te bestuderen door antigen presenterende cellen om T-cellen
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
DOI:

06:57 min

September 18, 2016

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Chapitres

  • 00:05Titre
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

Summary

Traduction automatique

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

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