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Travailler avec des cellules Auditory HEI-OC1
Journal JoVE
Biologie
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Journal JoVE Biologie
Working with Auditory HEI-OC1 Cells
DOI:

08:56 min

September 03, 2016

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Chapitres

  • 00:05Titre
  • 01:09Resuscitiation of Frozen HEI-OC1 Cells
  • 02:38Subculture of HEI-OC1 Cells
  • 04:14Drug Cytotoxicity Studied with HEI-OC1 Cells
  • 04:57Electrophysiology Experiments with HEI-OC1 Cells
  • 06:14Results: Viability, Death, and Prestin Expression in HEI-OC1 Cells and NP-HEI-OC1 Cells and NLC Studies
  • 08:19Conclusion

Summary

Traduction automatique

House Ear Institute-Organ of Corti 1 (HEI-OC1) is one of the few mouse auditory cell lines currently available for research purposes. This protocol describes how to work with HEI-OC1 cells to investigate the cytotoxic effects of pharmacological drugs as well as functional properties of inner ear proteins.

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