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Chapitres
- 00:05Titre
- 00:48Hydrogel Formation and Photopatterning
- 03:46Visualizing and Quantification of Photopatterning
- 06:18Encapsulating Cells and Photopatterning in the Presence of Cells
- 07:16Determining the Viability and Metabolic Activity of Encapsulated Cells
- 08:21Results: Quantitative and Qualitative Assessment of Photopatterning and Cell Viability
- 09:44Conclusion
We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.
