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Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

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Biochimie

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Journal JoVE Biochimie

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

DOI:

10.3791/54488-v

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10:39 min

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November 28, 2016

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Danielle L. Michell*¹, Ryan M. Allen*¹, Stuart R. Landstreet, Shilin Zhao, Cynthia L. Toth, Quanhu Sheng, Kasey C. Vickers²

¹Department of Medicine,Vanderbilt University School of Medicine, ²Center for Quantitative Sciences,Vanderbilt University School of Medicine, ³Department of Cancer Biology,Vanderbilt University School of Medicine

Chapitres

00:05Titre
01:13Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 1
03:07Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 2
04:31Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 3
06:05Fast-protein Liquid Chromatography (FPLC) for HDL Purification
08:05Results: Importance of DGUC to Procedure and Small RNA Library Analysis
09:24Conclusion

Summary

Traduction automatique

This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.

Tags

Keywords: HDL Lipoprotein Small RNA Non-coding RNA Density Gradient Ultracentrifugation Biomarker Cardiometabolic Disease High-throughput Sequencing Real-time PCR

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