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Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
Journal JoVE
Biologie
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Journal JoVE Biologie
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

DOI:
10.3791/54511-v

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08:24 min

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September 14, 2016

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, ,
1Chemotaxis Signal Section, Laboratory of Immunogenetics,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Chapitres

  • 00:05Titre
  • 01:08Cell Mobility Analysis Device Assembly
  • 03:11Cell Mobility Analysis
  • 06:00Results: Representative Cell Mobility Device Chemotaxis Analysis
  • 07:15Conclusion

Summary

Traduction automatique

Traduction automatique

Visual chemotaxis assays are essential for a better understanding of how eukaryotic cells control chemoattractant-mediated directional cell migration. Here, we describe detailed methods for: 1) real-time, high-resolution monitoring of multiple chemotaxis assays, and 2) simultaneously visualizing the chemoattractant gradient and the spatiotemporal dynamics of signaling events in neutrophil-like HL60 cells.

Tags

Keywords: G Protein-coupled ReceptorChemotaxisSignaling EventsNeutrophil-like HL60 CellsMicrofluidicsCell Mobility Analysis DeviceRPMI 1640 MediumBSACell BiologistsSimultaneous MonitoringReal-time Visualization

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