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In Situ Labeling van mitochondriaal DNA-replicatie in Drosophila volwassen Eierstokken van Edu kleuring
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Génétique
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Journal JoVE Génétique
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
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In Situ Labeling van mitochondriaal DNA-replicatie in Drosophila volwassen Eierstokken van Edu kleuring

DOI:
10.3791/54516-v

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10:31 min

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October 15, 2016

•

,
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute,National Institutes of Health

Chapitres

  • 00:05Titre
  • 00:41Tissue Collection and Dissection
  • 02:21EdU Labeling, Fixation, and Permeabilization
  • 04:17EdU Detection
  • 05:55Antibody Labeling
  • 07:21Mounting and Imaging
  • 08:37Results: EdU Staining of Mitochondrial DNA Replication in Drosophila Ovaries
  • 10:06Conclusion

Summary

Traduction automatique

Traduction automatique

Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

Tags

Keywords: Mitochondrial DNA ReplicationDrosophilaAdult OvariesEdU StainingMitochondrial BiogenesisMitochondrial DNA InheritanceIn Situ LabelingDrosophila MediumAphidicolinEdUOvary DissectionOvariolesFluorescent Dye Penetration

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