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1Davee Department of Neurology and Clinical Neurosciences,Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery,Northwestern University, 3Department of Pharmacology,Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences,Northwestern University, 5Department of Physiology,Feinberg School of Medicine, Northwestern University
Chapitres
- 00:05Titre
- 01:08High Throughput Screen
- 03:07Confirmation of Hits Using TAMRA-tagged HCN1 Peptide
- 04:33Dose-response Validation of Bead-based Proximity Assay
- 07:15Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions
- 09:14Conclusion
The interaction between HCN channels and their auxiliary subunit has been identified as a therapeutic target in Major Depressive Disorder. Here, a fluorescence polarization-based method for identifying small molecule inhibitors of this protein-protein interaction, is presented.
