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Chapitres
- 00:05Titre
- 00:35Dilution Antigens and Generating Antigen Microarrays
- 03:21Probe Antigen Microarrays with Diluted Sera
- 06:02Scanning Antigen Microarrays and Exporting Data
- 07:40Results: Detection of IgG and IgM Autoantibodies using Two-color Microarrays
- 09:29Conclusion
We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.
