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מדידת טיטר נוגדנים עיכוב Neuraminidase שפעת ידי enzyme-linked Assay לקטינים
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
DOI:

08:11 min

September 06, 2016

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Chapitres

  • 00:05Titre
  • 00:39Determination of the Amount of Neuraminidase (NA) to Use in Enzyme-linked Lectin Assay (ELLA)
  • 04:10Enzyme-linked Lectin Assay (ELLA)
  • 06:15Results: Determination of the Neuraminidase Inhibition Titer
  • 07:40Conclusion

Summary

Traduction automatique

We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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