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Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Journal JoVE
Biologie
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Journal JoVE Biologie
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

DOI:
10.3791/54586-v

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07:42 min

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September 17, 2016

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,
1Institute for Molecular Plant Sciences,University of Edinburgh

Chapitres

  • 00:05Titre
  • 00:39Protoplast Isolation
  • 03:16Protoplast Transfection
  • 05:03Imaging and Data Analysis
  • 05:48Results: Detection of Altered Circadian Rhythms in Arabidopsis Protoplasts
  • 07:02Conclusion

Summary

Traduction automatique

Traduction automatique

The circadian clock regulates about a third of the Arabidopsis transcriptome, but the percentage of genes that feed back into timekeeping remains unknown. Here we visualize a method to rapidly assess circadian phenotypes in any mutant line of Arabidopsis using luminescent imaging of a circadian reporter transiently expressed in protoplasts.

Tags

ArabidopsisProtoplastsCircadianLuminescentReporterCircadian PeriodCircadian BiologyGene FeedbackTransgenicEnzyme SolutionEpidermisMesophyll CellsW5 SolutionHemocytometerMMg Solution

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