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<em> In Vitro</em> Модель гематоэнцефалического барьера Использование импедансной спектроскопии: основноевнимание- Т-клеток-эндотелиальной клеточное взаимодействие
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
DOI:

10:17 min

December 08, 2016

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Chapitres

  • 00:05Titre
  • 01:57Harvesting MBMECs
  • 03:52Setting Up and Performing TEER Measurement
  • 07:18Results: Changes in TEER as a Primary Measure of MBMEC Dysfunction upon Interaction with T Cells
  • 09:01Conclusion

Summary

Traduction automatique

Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

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