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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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Immunologie et infection
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Journal JoVE Immunologie et infection
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

DOI:
10.3791/54615-v

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08:17 min

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October 30, 2016

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, , ,
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology,Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy,Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology,Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine,University of Oslo

Chapitres

  • 00:05Titre
  • 01:01NK Cell Isolation
  • 02:20NK Cell Stimulation
  • 03:52Surface and Intracellular Staining
  • 06:04Results: Representative Healthy Donor NK Cell Stimulation Analysis
  • 07:03Conclusion

Summary

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Traduction automatique

A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.

Tags

Keywords: Flow CytometryNK Cell FunctionsNatural Killer CellsViral InfectionsMalignant DiseasesPediatricsImmunodeficient PatientsNK Cell IsolationEDTA Peripheral Whole BloodNK Cell StimulationK562 Tumor CellsIL-2IL-15

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