Journal
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ile Enfeksiyon sırasında boşaltılması Lenf düğümleri içine Fare Cilt Dendritik Hücre Göç Eğitim Bir KAKE tabanlı Testi<em> Mycobacterium bovis</em> Basil Calmette-Guerin
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
DOI:

07:14 min

October 09, 2016

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Chapitres

  • 00:05Titre
  • 00:56Bacille Calmette-Guérin (BCG) and CFSE Injection
  • 02:08Popliteal Lymph Node (pLN) Harvest, Single Cell Suspension Preparation and Cell Surface Staining
  • 04:05Results: Migratory Skin Dendritic Cell Draining LN Relocation after BCG Footpad Infection
  • 06:04Conclusion

Summary

Traduction automatique

An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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