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Transduction-Transplantation Maus-Modell der myeloproliferativen Neoplasien
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Transduction-Transplantation Mouse Model of Myeloproliferative Neoplasm
DOI:

08:12 min

December 22, 2016

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Chapitres

  • 00:05Titre
  • 01:08Transduce Donor Bone Marrow Cells
  • 04:56Transplant Donor Cells into Recipient Mice
  • 06:43Results: Reconstitution of Hematopoietic Cells in Mice Using Transduction-Transplantation
  • 07:32Conclusion

Summary

Traduction automatique

This manuscript provides a description of the methodology used to establish transduction-transplantation mouse models. A detailed account is given of technical errors to avoid when performing bone marrow transplants. A clear understanding should be gained of the importance of high viral titer, transfection/transduction, and irradiation.

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