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Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

DOI:
10.3791/54633-v

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09:13 min

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October 24, 2016

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, , , , , ,
1Department of Applied Biochemistry,Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology,Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering,Institute of Biotechnology, Berlin University of Technology

Chapitres

  • 00:05Titre
  • 01:08Recellularization of Extracellular Matrix (ECM) of a Rat Liver
  • 04:28Transduction of the Recellularized Rat Liver
  • 05:59Assessment of the Recellularized Transduced Rat Liver
  • 07:14Results: Recellularization Assessment and Transduction Efficiency
  • 08:39Conclusion

Summary

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Traduction automatique

The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.

Tags

Keywords: 3D Liver ModelHepG2 Cell LineViral VectorsGene TransferDecellularized Rat Liver ScaffoldBioreactor SystemRecellularizationAnimal WelfareCell CultureHepatocellular Carcinoma

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