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Utarbeidelse av ekstracellulær matrix protein fiber for Brillouin Spectroscopy
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Utarbeidelse av ekstracellulær matrix protein fiber for Brillouin Spectroscopy

DOI:
10.3791/54648-v

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07:19 min

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September 15, 2016

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, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

Chapitres

  • 00:05Titre
  • 00:46Rat Tail Collagen Fiber Extraction
  • 02:16Bovine Nuchal Ligament Elastin Fiber Extraction
  • 03:44Reflective Substrate Fiber Mounting, Brillouin Experiment Setup and Fiber Spectra Acquisition
  • 04:55Results: Representative Micromechanics of Collagen and Elastin Fibers
  • 06:50Conclusion

Summary

Traduction automatique

Traduction automatique

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

Tags

Keywords: Extracellular MatrixCollagenElastinBrillouin SpectroscopyTendonChondroitinase ABCStreptomyces HyaluronidaseTrypsinBovine Nuchal LigamentSodium HydroxideWater Bath

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