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Sabit Görüntüleme prometafaz ve Metafaz Mayoz ve I Teknikleri<em> Drosophila</em> Oositler
Journal JoVE
Biologie
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Journal JoVE Biologie
Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes
DOI:

09:11 min

October 31, 2016

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Chapitres

  • 00:05Titre
  • 00:36Collection of Late-stage Drosophila Oocytes
  • 02:21Fixation of Oocyte Tissue and Membrane Removal (“Rolling”)
  • 04:09Antibody Staining of Drosophila Oocytes
  • 06:47Results: Visualization of Prophase and Metaphase in Drosophila Oocytes
  • 08:15Conclusion

Summary

Traduction automatique

We present protocols for the collection, preparation, and imaging of mature Drosophila oocytes. These methods allow the visualization of chromosome behavior and spindle assembly and function during meiosis.

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