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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

DOI:
10.3791/54726-v

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06:45 min

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November 21, 2016

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, ,
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine,Iowa State University, 2MRC Centre for Inflammation Research,University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University

Chapitres

  • 00:05Titre
  • 00:42Neutrophil Isolation
  • 03:41DNA Release Assay
  • 04:44Résultats
  • 05:42Conclusion

Summary

Traduction automatique

Traduction automatique

Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.

Tags

Neutrophil Extracellular TrapFluorescence AssayCanineNeutrophil IsolationAgonistImmunologyVeterinary MedicineCell DeathCell SeparationDextranPolysucroseSodium DiatrizoateErythrocyte LysisPBS

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