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Estudiar el Sistema de Control de Calidad de la Proteína<em> D. discoideum</em> Uso de imágenes de células vivas con temperatura controlada
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
DOI:

06:09 min

December 02, 2016

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Chapitres

  • 00:05Titre
  • 00:35Cell Preparation
  • 01:19Imaging
  • 02:53Image Analysis
  • 04:05Results: Behavior of Aggregation-prone Prion-like Proteins in D. discoideum during Heat Stress
  • 05:25Conclusion

Summary

Traduction automatique

The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.

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