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Den Indirekte Neuron-astrocyte coculture analyse: En<em> In Vitro</em> Oppsett for detaljert undersøkelse av Neuron-gliaceller interaksjoner
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Journal JoVE Neurosciences
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Den Indirekte Neuron-astrocyte coculture analyse: En<em> In Vitro</em> Oppsett for detaljert undersøkelse av Neuron-gliaceller interaksjoner

DOI:
10.3791/54757-v

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11:08 min

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November 14, 2016

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, , ,
1Department of Cell Morphology and Molecular Neurobiology, Faculty of Biology and Biotechnology,Ruhr-University

Chapitres

  • 00:05Titre
  • 02:01Preparation and Cultivation of Primary Cortical Astrocytes
  • 05:19Preparing Astrocytes for the Indirect Coculture
  • 06:48Preparation of Primary Hippocampal Neurons
  • 09:03Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
  • 10:01Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokollen beskriver indirekte nevron-astrocyte coculture for compartmentalized analyse av nevron-gliaceller interaksjoner.

Tags

Keywords: Indirect Neuron-astrocyte Coculture AssayNeuron-glia InteractionsSynapse FormationAstrocyte SecretomePrimary AstrocytesPrimary Embryonic NeuronsPermeable Membrane

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