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High-resolution Optical Mapping of the Mouse Sino-atrial Node
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Journal JoVE Biologie
High-resolution Optical Mapping of the Mouse Sino-atrial Node

High-resolution Optical Mapping of the Mouse Sino-atrial Node

DOI:
10.3791/54773-v

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11:07 min

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December 02, 2016

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,
1Department of Medicine,University of Wisconsin-Madison School of Medicine and Public Health

Chapitres

  • 00:05Titre
  • 00:54Heart Removal and Langendorff Perfusion
  • 02:28Optical Mapping of Sino-atrial Node (SAN) from Langendorff-perfused Whole Heart
  • 05:11Optical Mapping of the SAN from the Isolated Atrial Preparation
  • 08:22Results: Representative SAN Optical Mapping Analyses
  • 10:01Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol for optical mapping of electrical activity from the mouse right atrium and especially the sino-atrial node, at a high spatial and temporal resolution.

Tags

Keywords: Optical MappingSinoatrial NodeLangendorff-perfused HeartIsolated Atrial PreparationPacemaker AbnormalitiesSick Sinus SyndromeAtrial ArrhythmiasAnesthesiaThoracotomyAortic CannulationPerfusionPressure MonitoringOptical Mapping Setup

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