/

Immunologie et infection

/

人全血中NK细胞杀伤活性的流式细胞仪分析

Journal JoVE
Immunologie et infection

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Immunologie et infection

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Please note that all translations are automatically generated. Click here for the English version.

人全血中NK细胞杀伤活性的流式细胞仪分析

DOI:

10.3791/54779-v

•

08:14 min

•

March 17, 2017

•

Jennifer E. McBride, Mary P. Meaney, Casey John, David C. Nieman, Renaud F. Warin

¹Center for Excellence in Post-Harvest Technologies,North Carolina A&T State University, North Carolina Research Campus, ²Human Performance Laboratory,Appalachian State University, North Carolina Research Campus

Chapitres

00:05Titre
00:44Automated NK Cell Separation
02:17Cytotoxicity Assay Sample Preparation
03:36Imaging Flow Cytometer Acquisition and Analysis
06:25Results: Representative NK Cell Lytic Activity Analyses
07:41Conclusion

Summary

Traduction automatique

This work describes an advanced workflow for the accurate and fast determination of NK (Natural Killer) cell count and NK cell cytotoxicity in human blood samples.

Tags

Flow Cytometry Natural Killer (NK) Cells Cytotoxicity Whole Blood Cell Separation K562 Cells Propidium Iodide Immunology

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

的自然杀伤细胞免疫突触上的玻璃支承平面脂双层超分辨率成像

对于纳米颗粒疫苗接种后的分析抗原特异性CD8 + T细胞应答整体动物成像和流式细胞技术

分离和流式细胞仪分析从缺血小鼠脑免疫细胞

分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析

一个简单的流式细胞仪法测量葡萄糖的摄取和葡萄糖转运表达单核细胞亚群的全血

粒子结合Bet v 1的变应原PM10的流式细胞分析

人类宫颈的γδT细胞的分离和流式细胞仪分析

葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率

大鼠外周全血细胞免疫表型和因子生成的单细胞分析

全血流式细胞术分析人单核细胞亚群的表征

Read Article