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Chapitres
- 00:05Titre
- 00:57Single-molecule FRET (smFRET) Measurements with Total Internal Reflection Fluorescence (TIRF) Microscopy
- 05:19Determination of Mean FRET Efficiency
- 06:45Determination of Credible Volumes with the Fast Nano-positioning System (Fast-NPS)
- 10:09Results: smFRET Fast Nano-positioning System Modeling of Archaeal RNA Polymerase Open Promotor Complex
- 11:42Conclusion
We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.
Tags
Keywords:
Single-molecule FRETFast Nano-positioning SystemStructural BiologyThree-dimensional Probability DistributionProtein DatabaseFluorescence MeasurementsFlow ChamberSample HolderNeutravidinTotal Internal Reflection Fluorescence MicroscopeStage Piezo-motorTIRF-microscopeImmersion OilImmersion LiquidCamera ParametersFile PathAuto-increment Parameters
