Journal
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Biochimie
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Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

DOI:
10.3791/54792-v

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12:21 min

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November 27, 2016

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, ,
1Vollum Institute,Oregon Health & Science University, 2Graduate Group in Biophysics,University of California, San Francisco, 3Howard Hughes Medical Institute,Oregon Health & Science University

Chapitres

  • 00:05Titre
  • 00:47Transfection of Adherent HEK293S GnTI-cells for Thermostability Screen
  • 02:24Scintillation Proximity-based Thermostability Screen with Citalopram
  • 04:18Expression of the Human Serotonin Transporter in HEK293S GnTI-cells
  • 05:24Affinity-purification of the Serotonin Transporter for Immunization and Crystallization
  • 07:12Formation of Transporter-antibody Complexes and Separation by Size-exclusion Chromatography
  • 08:21Crystallization of Transporter-antibody Complexes by Hanging Drop
  • 10:01Results: Thermostabilization and Crystallization of the Serotonin Transporter in an Antidepressant-bound State
  • 11:25Conclusion

Summary

Traduction automatique

Traduction automatique

This manuscript describes how to screen for thermostabilizing mutations, purify the human serotonin transporter, generate high affinity antibodies, and crystallize the serotonin transporter-antibody complex bound to the antidepressant drug S-citalopram. This protocol can be adapted to the study of other challenging membrane transporters, receptors, and channels.

Tags

Keywords: Serotonin TransporterS-citalopramThermostabilizationExpressionPurificationCrystallizationMembrane ProteinsDrug TargetsNeurotransmitter TransportersReceptorsChannelsSoluble ProteinsHEK293S GnTIDNA TransfectionCitalopramC12MCHSProtease InhibitorsTritiated CitalopramBSAHis-tag AffinitySPA BeadsSertraline

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