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Chapitres
- 00:05Titre
- 01:06Preparation of PEGylated Antibody (PEG-Ab) Solution
- 02:25Prepare UV-Vis/Raman Probes
- 04:57Immunoassay Plate Preparation
- 06:08Functionalizing the Immunoassay Plate
- 07:18Test Assay Sensitivity by UV-Vis and Raman Spectroscopy
- 07:58Results: Determining the Sensitivity of UV-Vis and Raman Spectroscopy
- 08:39Conclusion
Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.
