Journal
/
Génétique
/
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

DOI:
10.3791/54810-v

•

04:51 min

•

November 14, 2016

•

,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Chapitres

  • 00:05Titre
  • 00:46Assembling the LED Illuminator
  • 01:52Blue Light Treatment
  • 03:46Results: Screen Mutation Rate
  • 04:27Conclusion

Summary

Traduction automatique

Traduction automatique

Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.

Tags

OptogeneticRandom MutagenesisHistone-miniSOGC. ElegansLEDReactive Oxygen SpeciesROS-generating ProteinsMutagenesis ProtocolBlue LightDigital Function GeneratorPhotometerCopper ChlorideSine WaveYoung Adult Hermaphrodites

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Manipulation of Ploidy in Caenorhabditis elegans

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Read Article