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En In Vitro -modellen for å undersøke mobilnettet transformasjon av Kaposis sarkom Herpesvirus
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Journal JoVE Biologie
An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

En In Vitro -modellen for å undersøke mobilnettet transformasjon av Kaposis sarkom Herpesvirus

DOI:
10.3791/54828-v

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09:53 min

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August 25, 2017

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, , , ,
1Division of Pediatric Infectious Diseases,University of Minnesota Medical School, 2Vaccine and Gene Therapy Institute,Oregon Health and Science University

Chapitres

  • 00:05Titre
  • 01:09KSHV Stock Preparation
  • 03:15Transduction of Endothelial Cells with E6 and E7 Papillomavirus Genes
  • 05:41Infection of Transduced Endothelial Cells with KSHV
  • 07:38Results: Phenotypic Changes in KSHV-infected Endothelial Cells
  • 09:15Conclusion

Summary

Traduction automatique

Traduction automatique

Kaposis sarkom (KS) er en svulst forårsaket av infeksjon med kreftfremkallende viruset menneskelig herpesvirus-8/KS herpesvirus (HHV-8/KSHV). Endothelial celle kultur modellen beskrevet her er unikt tilpasset for å studere mekanismer som KSHV forvandler vertsceller.

Tags

Keywords: In Vitro ModelCellular TransformationKaposi Sarcoma HerpesvirusKSHVEndothelial CellsMalignant TransformationViral OncogenesisKaposi SarcomaPathological AngiogenesisBCBL-1 CellsPMAViral Particle IsolationUltracentrifugation

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