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1Department of Pediatrics, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, 2Department of Pediartics, Omaha Children’s Hospital and Medical Center,University of Nebraska Medical Center, 3Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, 4Indiana Biosciences Research Institute
Chapitres
- 00:05Titre
- 00:42Serum DNA Extraction
- 03:01Bisulfite Conversion
- 04:59Multiplex Digital PCR
- 08:00Results: Differentially Methylated INS DNA Measurements
- 09:25Conclusion
Islet β cell death precedes development of type 1 diabetes, and detecting this process may allow for early therapeutic intervention. Here, we provide a detailed description of how to measure differentially methylated INS DNA species in human serum as a biomarker of β cell death.
