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Højt gennemløb, realtid, Dual-udlæsning Test af Intracellulær antimikrobielle aktivitet og eukaryote celle cytotoksicitet
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
DOI:

09:09 min

November 16, 2016

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Chapitres

  • 00:05Titre
  • 01:30Real Time Intracellular Growth and Eukaryotic Cell Cytotoxicity Assay
  • 03:14Preparing Luminescent or Fluorescent Legionella pneumophila and Macrophage Infection
  • 04:49Assay Readout, and Single and Multi-dimensional Dose-response Testing and Data Interpretation
  • 06:09Results: Simultaneous Real-time Assay Results for Intracellular Bacterial Growth and Eukaryotic Cell Cytotoxicity
  • 07:41Conclusion

Summary

Traduction automatique

A high throughput, real-time assay was developed to simultaneously identify (1) eukaryotic cell-penetrant antimicrobials targeting an intracellular bacterial pathogen, and (2) assess eukaryotic cell cytotoxicity. A variation on the same technology was thereafter combined with digital dispensing technology to enable facile, high-resolution, dose-response, and two- and three-dimensional synergy studies.

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