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间质干细胞培养和交付自体条件:用于骨科应用程序的智能方法
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
DOI:

10:30 min

December 08, 2016

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Chapitres

  • 00:05Titre
  • 00:57Preparation of Autologous Serum
  • 02:20Isolation of hMSCs from Bone Marrow
  • 03:42Culture of hMSCs in the Presence of Autologous Serum
  • 05:19Osteogenic Pre-induction and Recovery of hMSCs
  • 06:20Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
  • 07:30Results: hMSCs/Fibrin Clot Generation and Application
  • 09:10Conclusion

Summary

Traduction automatique

培养人骨髓间充质基质细胞(hMSCs)与自体血清,降低排斥异种材料等负面影响的风险。它也允许对中胚层祖细胞的子集,这可以提供新鲜的hMSCs的恢复。在自体纤维蛋白凝块嵌入的hMSCs可轻松处理和有效的手术植入。

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