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Chapitres
- 00:05Titre
- 00:59Amplification of the Chloramphenicol Acetyltransferase (CAT) Cassette from pBAD33 Plasmid Using Oligomers Designed for Homologous Pairing
- 02:38Generation of pOX38-Tc Δgene::Cm Strains
- 04:26Conjugative Mating Assay from XK1200 Donors to MC4100 Recipients
- 08:38Results: Transfer Gene Knockout and Recovery of Conjugative DNA Transfer
- 09:45Conclusion
Here, we present a protocol to knockout a gene of interest involved in plasmid conjugation and subsequently analyze the impact of its absence using mating assays. The function of the gene is further explored to a specific region of its sequence using deletion or point mutations.
