JoVE Journal
Developmental Biology
Developmental Biology
Ce contenu est en libre accès.
Chapitres
résumé
We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.