Um protocolo para usar a transferência de Förster Ressonância Energia (FRET) Biossensores -force medir forças mecânicas em todo o Complexo LINC Nuclear
A number of FRET-based force biosensors have recently been developed, enabling the protein-specific resolution of intracellular force. In this protocol, we demonstrate how one of these sensors, designed for the linker of the nucleoskeleton-cytoskeleton (LINC) complex protein Nesprin-2G can be used to measure actomyosin forces on the nuclear LINC complex.