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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Journal JoVE
Génétique
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Journal JoVE Génétique
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

DOI:
10.3791/54952-v

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09:08 min

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January 13, 2017

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, , ,
1Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology,Universität Siegen

Chapitres

  • 00:05Titre
  • 00:54Identification of a Natural Product with Antibiotic Property
  • 03:54Propose Biosynthetic Model of the New Isolated Compound
  • 05:02Identification of the Biosynthetic Gene Cluster
  • 06:34Verification of the Biosynthetic Gene Cluster
  • 07:40Results: HPLC Analysis of S. diastatochromogenes with Inactivated pokPI Gene
  • 08:07Conclusion

Summary

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Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.

Tags

Keywords: Natural ProductBiosynthetic Gene ClusterPolyketomycinStreptomyces DiastatochromogenesAntibiotic ActivityGenome MiningStreptomycesAntibiotic ProducerBiosynthetic PathwayCompound ExtractionHPLCDisc Diffusion AssayTest StrainAntibiotic

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