Journal
/
Génétique
/
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

DOI:
10.3791/54969-v

•

08:48 min

•

December 21, 2016

•

, , , , ,
1Department of Occupational and Environmental Health,University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences,University of Alabama at Birmingham, 3Division of Radiation Health,University of Arkansas for Medical Sciences

Chapitres

  • 00:05Titre
  • 00:35Particle Exposure
  • 02:12Cell Treatment with Particulate Matter
  • 03:21Cell Harvest, Hypotonic Treatment, and Fixation
  • 05:35Slide Cleaning and Metaphase Spread Preparation
  • 07:12Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
  • 08:08Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes techniques for the quantification and characterization of chromosomal aberrations in vitro in RAW264.7 mouse macrophages after treatment with ambient air particulate matter.

Tags

Chromosomal AberrationsParticulate MatterIn VitroAir PollutionCell CultureGenotoxicityMetaphase ArrestTrypsinCell SuspensionCell DensityCell IncubationPBSColcemid

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

In Vitro Transcription Assays and Their Application in Drug Discovery
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

CRISPR-Mediated Reorganization of Chromatin Loop Structure
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Measuring Exercise Levels in Drosophila melanogaster Using the Rotating Exercise Quantification System (REQS)
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
check_url/fr/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Read Article