JoVE Journal
Developmental Biology
Developmental Biology
Ce contenu est en libre accès.
Chapitres
résumé
We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.