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Culture of Murine embryonale metatarsals: en fysiologisk modell av endochondral Forbening
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Chapitres
- 00:05Titre
- 00:51Dissection Preparation
- 01:47Murine Embryonic Metatarsal Dissection and Culture
- 04:57Results: A Tool for Understanding Endochondral Ossification
- 06:20Conclusion
We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.
