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应用改良袖套技术的小鼠胰腺移植模型

Journal JoVE
Médecine
Author Produced

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Journal JoVE Médecine

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

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应用改良袖套技术的小鼠胰腺移植模型

DOI:

10.3791/54998-v

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13:19 min

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December 16, 2017

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Benno Cardini*¹, Rupert Oberhuber*¹, Sven R Hein, Rebecca Eiter, Martin Hermann, Markus Kofler³, Stefan Schneeberger, Gerald Brandacher⁴, Manuel Maglione

¹Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery,Medical University Innsbruck, ²Department of Anesthesiology and Critical Care Medicine,Medical University Innsbruck, ³Department of Cardiac Surgery,Medical University Innsbruck, ⁴Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory,Johns Hopkins University School of Medicine

Chapitres

00:00Titre
00:22Pancreas procurement
05:24Recipient preparation
09:36Vascular anastomosis
11:41Representative results
12:48Conclusion

Summary

Traduction automatique

在腹部实体器官移植中, 胰腺移植容易发生严重的缺血再灌注损伤-相关的移植损伤, 最终导致早期的移植丢失。该协议描述了一个模型的小鼠胰腺移植使用 non-suture 袖技术, 最适合于分析这些早期的, 有害的损害。

Tags

Keywords: Mouse Model Pancreas Transplantation Modified Cuff Technique Ketamine Xylazine Midline Laparotomy Heparin Abdominal Aorta Common Bile Duct Portal Vein Renal Vein Celiac Trunk Aortic Patch Perfusion Solution Duodenum Ligament Of Treitz

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