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激光显微切割RNA测序实验设计:从玉米叶片发展的经验分析
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
DOI:

10:08 min

March 05, 2017

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Chapitres

  • 00:05Titre
  • 00:45Tissue Fixation and Processing
  • 03:03Sectioning and Slide Preparation
  • 04:57Microdissection of Blade, Ligule, and Sheath Samples from Plastochron 7 Leaf Primordia
  • 07:48Results: Laser Microdissection RNA Sequencing Identifies Differentially Expressed Genes in Maize Leaf
  • 09:38Conclusion

Summary

Traduction automatique

Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.

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