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inoculant<em> Anopheles gambiae</em> Les moustiques avec perles à induire et mesurer la réponse immunitaire mélanisation
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
DOI:

08:24 min

January 12, 2017

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Chapitres

  • 00:05Titre
  • 00:44Preparation of Saline Solution and Bead Selection with Capillary
  • 02:21Mosquito Handling and Inoculation
  • 03:52Mosquito Dissection and Bead Photography
  • 04:59Picture Analysis: Bead Melanization
  • 06:05Results: Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
  • 07:39Conclusion

Summary

Traduction automatique

Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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