Journal
/
/
Immagini dal vivo per studiare Microtubulo instabilità dinamica nei tumori al seno taxani resistente
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
DOI:

06:02 min

February 20, 2017

, ,

Chapitres

  • 00:05Titre
  • 00:37Cell Culture and Seeding
  • 01:43GFP-tagged α-tubulin Expression
  • 03:34Image Processing and Data Analysis
  • 04:27Results: Representative Effects of Docetaxel in the Microtubule Dynamics of MCF-7TXT and MCF-7CC Cells
  • 05:05Conclusion

Summary

Traduction automatique

In this paper, we report a protocol describing an in vivo method to measure microtubule dynamic instability in docetaxel-resistant breast cancer cells (MCF-7TXT). In this method, a deconvolution microscopy imaging system is used to detect the expression of GFP-tubulin in target cells.

Vidéos Connexes

Read Article