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Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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Journal JoVE Biologie du développement
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

DOI:
10.3791/55028-v

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10:04 min

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January 08, 2017

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1Division of Morphological Science, Biomedical Research Center,Saitama Medical University, 2Department of Pathology, International Medical Center,Saitama Medical University

Chapitres

  • 00:05Titre
  • 00:51Induction and Establishment of Tetraploid Cells from Diploid Fibroblasts without the Shake-off Method
  • 01:52Induction and Establishment of Tetraploid Cells from Diploid Fibroblasts with the Shake-off Method
  • 04:01Examination of DNA Ploidy
  • 06:30Preparation for Chromosome Counts and Karyotype Analysis
  • 08:04Results: Establishment of Proliferative Tetraploid Cells
  • 09:28Conclusion

Summary

Traduction automatique

Traduction automatique

Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.

Tags

Tetraploid CellsHuman FibroblastsCarcinogenesis ResearchAneuploid CellsCell CultureDemecolcineMitotic CellsCell CountingCell Reseeding

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